• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast.

The roles of two kinesin-related proteins, Kip2p and Kip3p, in microtubule function and nuclear migration were investigated. Deletion of either gene resulted in nuclear migration defects similar to those described for dynein and kar9 mutants. By indirect immunofluorescence, the cytoplasmic microtubules in kip2Delta were consistently short or absent throughout the cell cycle. In contrast, in kip3Delta strains, the cytoplasmic microtubules were significantly longer than wild type at telophase. Furthermore, in the kip3Delta cells with nuclear positioning defects, the cytoplasmic microtubules were misoriented and failed to extend into the bud. Localization studies found Kip2p exclusively on cytoplasmic microtubules throughout the cell cycle, whereas GFP-Kip3p localized to both spindle and cytoplasmic microtubules. Genetic analysis demonstrated that the kip2Delta kar9Delta double mutants were synthetically lethal, whereas kip3Delta kar9Delta double mutants were viable. Conversely, kip3Delta dhc1Delta double mutants were synthetically lethal, whereas kip2Delta dhc1Delta double mutants were viable. We suggest that the kinesin-related proteins, Kip2p and Kip3p, function in nuclear migration and that they do so by different mechanisms. We propose that Kip2p stabilizes microtubules and is required as part of the dynein-mediated pathway in nuclear migration. Furthermore, we propose that Kip3p functions, in part, by depolymerizing microtubules and is required for the Kar9p-dependent orientation of the cytoplasmic microtubules.

Pubmed ID: 9693366


  • Miller RK
  • Heller KK
  • Frisèn L
  • Wallack DL
  • Loayza D
  • Gammie AE
  • Rose MD


Molecular biology of the cell

Publication Data

August 30, 1998

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM-37739
  • Agency: NIGMS NIH HHS, Id: GM-52526

Mesh Terms

  • Base Sequence
  • Cell Cycle
  • Cell Nucleus
  • DNA Primers
  • Fluorescent Antibody Technique, Indirect
  • Fungal Proteins
  • Kinesin
  • Microtubule-Associated Proteins
  • Microtubules
  • Molecular Motor Proteins
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins