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A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation.

A number of transcriptional coactivator proteins have been identified as histone acetyltransferase (HAT) proteins, providing a direct molecular basis for the coupling of histone acetylation and transcriptional activation. The yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) complex requires the coactivator protein Gcn5 for HAT activity. Identification of protein subunits by mass spectrometry and immunoblotting revealed that the TATA binding protein-associated factors (TAF(II)s) TAF(II)90, -68/61, -60, -25/23, and -20/17 are integral components of this complex. In addition, TAF(II)68 was required for both SAGA-dependent nucleosomal HAT activity and transcriptional activation from chromatin templates in vitro. These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145.

Pubmed ID: 9674426

Authors

  • Grant PA
  • Schieltz D
  • Pray-Grant MG
  • Steger DJ
  • Reese JC
  • Yates JR
  • Workman JL

Journal

Cell

Publication Data

July 10, 1998

Associated Grants

  • Agency: PHS HHS, Id: 11823-02

Mesh Terms

  • Acetyltransferases
  • DNA-Binding Proteins
  • Fungal Proteins
  • Histone Acetyltransferases
  • Histones
  • Nucleosomes
  • Protein Kinases
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • TATA-Binding Protein Associated Factors
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription, Genetic