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A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation.

Cell | Jul 10, 1998

http://www.ncbi.nlm.nih.gov/pubmed/9674426

A number of transcriptional coactivator proteins have been identified as histone acetyltransferase (HAT) proteins, providing a direct molecular basis for the coupling of histone acetylation and transcriptional activation. The yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) complex requires the coactivator protein Gcn5 for HAT activity. Identification of protein subunits by mass spectrometry and immunoblotting revealed that the TATA binding protein-associated factors (TAF(II)s) TAF(II)90, -68/61, -60, -25/23, and -20/17 are integral components of this complex. In addition, TAF(II)68 was required for both SAGA-dependent nucleosomal HAT activity and transcriptional activation from chromatin templates in vitro. These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145.

Pubmed ID: 9674426 RIS Download

Mesh terms: Acetyltransferases | DNA-Binding Proteins | Fungal Proteins | Histone Acetyltransferases | Histones | Nucleosomes | Protein Kinases | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | TATA-Binding Protein Associated Factors | TATA-Box Binding Protein | Transcription Factor TFIID | Transcription Factors | Transcription, Genetic

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Associated grants

  • Agency: PHS HHS, Id: 11823-02

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BioGRID (Data, Interactions)

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