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Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo.

Molecular cell | Mar 28, 1998

We have identified a virus-activated factor (VAF) that binds to a regulatory element shared by different virus-inducible genes. We provide evidence that VAF contains two members of the interferon regulatory factor (IRF) family of transcriptional activator proteins (IRF-3 and IRF-7), as well as the transcriptional coactivator proteins p300 and CBP. Remarkably, VAF, as well as recombinant IRF-3 and IRF-7 proteins, binds very weakly to the interferon-beta (IFN-beta) gene promoter in vitro. However, in virus-infected cells, both proteins are recruited to the endogenous IFN-beta promoter as part of a protein complex that includes ATF-2/c-Jun and NF-kappa B. These observations provide a unique example of the coordinate activation of multiple transcriptional activator proteins and their highly cooperative assembly into a transcriptional enhancer complex in vivo.

Pubmed ID: 9660935 RIS Download

Mesh terms: Activating Transcription Factor 2 | Animals | Base Sequence | CHO Cells | CREB-Binding Protein | Cell Nucleus | Cricetinae | Cyclic AMP Response Element-Binding Protein | DNA | DNA-Binding Proteins | Enhancer Elements, Genetic | Fungal Proteins | Gene Expression Regulation, Viral | HeLa Cells | Humans | Interferon Regulatory Factor-3 | Interferon Regulatory Factor-7 | Interferon-beta | Leucine Zippers | Molecular Sequence Data | NF-kappa B | Nuclear Proteins | Phosphorylation | Recombinant Fusion Proteins | Respirovirus | Respirovirus Infections | Saccharomyces cerevisiae Proteins | Trans-Activators | Transcription Factors | Transcription, Genetic

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Associated grants

  • Agency: NIAID NIH HHS, Id: AI20642
  • Agency: NCI NIH HHS, Id: CA50661

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