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Geminin, an inhibitor of DNA replication, is degraded during mitosis.

We describe a novel 25 kDa protein, geminin, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin has a destruction box sequence and is ubiquitinated anaphase-promoting complex (APC) in vitro. In synchronized HeLa cells, geminin is absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). We propose that geminin inhibits DNA replication during S, G2, and M phases and that geminin destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Pubmed ID: 9635433

Authors

  • McGarry TJ
  • Kirschner MW

Journal

Cell

Publication Data

June 12, 1998

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Anaphase-Promoting Complex-Cyclosome
  • Animals
  • Cell Cycle Proteins
  • Cell Division
  • Cell Nucleus
  • Chromatin
  • DNA Replication
  • DNA, Complementary
  • Geminin
  • HeLa Cells
  • Humans
  • Ligases
  • Mitosis
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Ubiquitin-Protein Ligase Complexes
  • Ubiquitin-Protein Ligases
  • Ubiquitins
  • Xenopus Proteins
  • Xenopus laevis