RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor.
Calcitonin-gene-related peptide (CGRP) and adrenomedullin are related peptides with distinct pharmacological profiles. Here we show that a receptor with seven transmembrane domains, the calcitonin-receptor-like receptor (CRLR), can function as either a CGRP receptor or an adrenomedullin receptor, depending on which members of a new family of single-transmembrane-domain proteins, which we have called receptor-activity-modifying proteins or RAMPs, are expressed. RAMPs are required to transport CRLR to the plasma membrane. RAMP1 presents the receptor at the cell surface as a mature glycoprotein and a CGRP receptor. RAMP2-transported receptors are core-glycosylated and are adrenomedullin receptors.
Pubmed ID: 9620797 RIS Download
3T3 Cells | Adrenomedullin | Amino Acid Sequence | Animals | Biological Transport | Calcitonin | Calcitonin Gene-Related Peptide | Calcitonin Receptor-Like Protein | Cell Line | Cloning, Molecular | Cross-Linking Reagents | Cystic Fibrosis Transmembrane Conductance Regulator | Gene Expression | Glycosylation | Humans | Intracellular Signaling Peptides and Proteins | Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase | Membrane Proteins | Mice | Molecular Sequence Data | Peptides | RNA, Messenger | Receptor Activity-Modifying Protein 1 | Receptor Activity-Modifying Protein 2 | Receptor Activity-Modifying Proteins | Receptors, Adrenomedullin | Receptors, Calcitonin | Receptors, Calcitonin Gene-Related Peptide | Receptors, Peptide | Sequence Alignment | Tumor Cells, Cultured | Xenopus