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Yeast Ku as a regulator of chromosomal DNA end structure.

During telomere replication in yeast, chromosome ends acquire an S-phase-specific overhang of the guanosine-rich strand. Here it is shown that in cells lacking Ku, a heterodimeric protein involved in nonhomologous DNA end joining, these overhangs are present throughout the cell cycle. In vivo cross-linking experiments demonstrated that Ku is bound to telomeric DNA. These results show that Ku plays a direct role in establishing a normal DNA end structure on yeast chromosomes, conceivably by functioning as a terminus-binding factor. Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku has a distinct function when bound to telomeres.

Pubmed ID: 9563951

Authors

  • Gravel S
  • LarrivĂ©e M
  • Labrecque P
  • Wellinger RJ

Journal

Science (New York, N.Y.)

Publication Data

May 1, 1998

Associated Grants

None

Mesh Terms

  • Antigens, Nuclear
  • Binding Sites
  • Chromosomes, Fungal
  • DNA Helicases
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • G2 Phase
  • Genes, Fungal
  • Mitosis
  • Mutation
  • Nuclear Proteins
  • S Phase
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Telomerase
  • Telomere
  • Temperature
  • Transformation, Genetic