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A coactivator of pre-mRNA splicing.

The nuclear matrix antigen recognized by the monoclonal antibody (mAb) B1C8 is a novel serine (S) and arginine (R)-rich protein associated with splicing complexes and is named here SRm160 (SR-related matrix protein of 160 kD). SRm160 contains multiple SR repeats, but unlike proteins of the SR family of splicing factors, lacks an RNA recognition motif. SRm160 and a related protein SRm300 (the 300-kD nuclear matrix antigen recognized by mAb B4A11) form a complex that is required for the splicing of specific pre-mRNAs. The SRm160/300 complex associates with splicing complexes and promotes splicing through interactions with SR family proteins. Binding of SRm160/300 to pre-mRNA is normally also dependent on U1 snRNP and is stabilized by U2 snRNP. Thus, SRm160/300 forms multiple interactions with components bound directly to important sites within pre-mRNA. The results suggest that a complex of the nuclear matrix proteins SRm160 and SRm300 functions as a coactivator of pre-mRNA splicing.

Pubmed ID: 9531537

Authors

  • Blencowe BJ
  • Issner R
  • Nickerson JA
  • Sharp PA

Journal

Genes & development

Publication Data

April 1, 1998

Associated Grants

  • Agency: NIAID NIH HHS, Id: R01-AI32486
  • Agency: NCI NIH HHS, Id: R01-CA42063
  • Agency: NIGMS NIH HHS, Id: R01-GM34277

Mesh Terms

  • Amino Acid Sequence
  • Antigens, Nuclear
  • Bacterial Proteins
  • HeLa Cells
  • Humans
  • Interphase
  • Lymphoma, Large B-Cell, Diffuse
  • Metaphase
  • Molecular Sequence Data
  • Nuclear Matrix
  • Nuclear Matrix-Associated Proteins
  • Nuclear Proteins
  • RNA Precursors
  • RNA-Binding Proteins
  • Ribonucleoprotein, U1 Small Nuclear
  • Ribonucleoprotein, U2 Small Nuclear
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Spliceosomes
  • Tumor Cells, Cultured