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Testicular degeneration in Bclw-deficient mice.

To identify genes required for mammalian spermatogenesis, we screened lines of mutant mice created using a retroviral gene-trap system for male infertility. Homozygous ROSA41 male mice exhibit sterility associated with progressive testicular degeneration. Germ-cell defects are first observed at 19 days post-natal (p19). Spermatogenesis is blocked during late spermiogenesis in young adults. Gradual depletion of all stages of germ cells results in a Sertoli-cell-only phenotype by approximately six months of age. Subsequently, almost all Sertoli cells are lost from the seminiferous tubules and the Leydig cell population is reduced. Molecular analysis indicates that the gene mutated is Bclw, a death-protecting member of the Bcl2 family. The mutant allele of Bclw in ROSA41 does not produce a Bclw polypeptide. Expression of Bclw in the testis appears to be restricted to elongating spermatids and Sertoli cells. Potential roles for Bclw in testicular function are discussed.

Pubmed ID: 9500547

Authors

  • Ross AJ
  • Waymire KG
  • Moss JE
  • Parlow AF
  • Skinner MK
  • Russell LD
  • MacGregor GR

Journal

Nature genetics

Publication Data

March 30, 1998

Associated Grants

  • Agency: NIGMS NIH HHS, Id: 5 T32 GM08367
  • Agency: NICHD NIH HHS, Id: HD-24875

Mesh Terms

  • Age Factors
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Follicle Stimulating Hormone
  • Homozygote
  • Immunohistochemistry
  • Infertility, Male
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Mutant Strains
  • Microscopy, Electron
  • Molecular Sequence Data
  • Proteins
  • Proviruses
  • Random Amplified Polymorphic DNA Technique
  • Sequence Analysis
  • Sertoli Cells
  • Spermatozoa
  • Testis
  • Tissue Distribution