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Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1.

The TATA binding protein (TBP) is a central component of the eukaryotic transcriptional machinery and is the target of positive and negative transcriptional regulators. Here we describe the cloning and biochemical characterization of an abundant human TBP-associated factor (TAF-172) which is homologous to the yeast Mot1 protein and a member of the larger Snf2/Swi2 family of DNA-targeted ATPases. Like Mot1, TAF-172 binds to the conserved core of TBP and uses the energy of ATP hydrolysis to dissociate TBP from DNA (ADI activity). Interestingly, ATP also causes TAF-172 to dissociate from TBP, which has not been previously observed with Mot1. Unlike Mot1, TAF-172 requires both TBP and DNA for maximal (approximately 100-fold) ATPase activation. TAF-172 inhibits TBP-driven RNA polymerase II and III transcription but does not appear to affect transcription driven by TBP-TAF complexes. As it does with Mot1, TFIIA reverses TAF-172-mediated repression of TBP. Together, these findings suggest that human TAF-172 is the functional homolog of yeast Mot1 and uses the energy of ATP hydrolysis to remove TBP (but apparently not TBP-TAF complexes) from DNA.

Pubmed ID: 9488487

Authors

  • Chicca JJ
  • Auble DT
  • Pugh BF

Journal

Molecular and cellular biology

Publication Data

March 19, 1998

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM47855
  • Agency: NIGMS NIH HHS, Id: GM55763

Mesh Terms

  • Adenosine Triphosphatases
  • Adenosine Triphosphate
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA Helicases
  • DNA, Complementary
  • DNA-Binding Proteins
  • Fungal Proteins
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Spodoptera
  • TATA-Binding Protein Associated Factors
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription, Genetic