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The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA.

GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The alpha subunit contains a DNA-binding domain that is a member of the ETS family, whereas the beta subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPalpha/beta ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2. 15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPalpha ETS domain binds to its core GGA DNA-recognition motif.

Pubmed ID: 9461436

Authors

  • Batchelor AH
  • Piper DE
  • de la Brousse FC
  • McKnight SL
  • Wolberger C

Journal

Science (New York, N.Y.)

Publication Data

February 13, 1998

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Ankyrins
  • Crystallography, X-Ray
  • DNA
  • DNA-Binding Proteins
  • Dimerization
  • GA-Binding Protein Transcription Factor
  • Hydrogen Bonding
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation
  • Protein Structure, Secondary
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-ets
  • Recombinant Proteins
  • Trans-Activators
  • Transcription Factors