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Rack1, a receptor for activated protein kinase C, interacts with integrin beta subunit.

The integrin beta subunit cytoplasmic domains are important for activation-dependent cell adhesion and adhesion-dependent signaling events. We report an interaction between integrin beta subunit cytoplasmic domain and Rack1, a Trp-Asp (WD) repeat protein that has been shown to bind activated protein kinase C. The Rack1-binding site on integrin beta 2 subunit resides within a conserved, membrane-proximal region. In the yeast two-hybrid assay, WD repeats five to seven of Rack1 (Rack1-WD5/7) interact with integrin beta 1, beta 2, and beta 5 cytoplasmic domain. In eukaryotic cells, Rack1 co-immunoprecipitates with at least two different beta integrins, beta 1 integrins in 293T cells and beta 2 integrins in JY lymphoblastoid cells. Whereas Rack1-WD5/7 binds integrins constitutively, the association of full-length Rack1 to integrins in vivo requires a treatment with phorbol esters, which promotes cell spreading and adhesion. These findings suggest that Rack1 may link protein kinase C directly to integrins and participate in the regulation of integrin functions.

Pubmed ID: 9442085

Authors

  • Liliental J
  • Chang DD

Journal

The Journal of biological chemistry

Publication Data

January 23, 1998

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Antigens, CD18
  • Binding Sites
  • Cell Adhesion
  • Humans
  • Molecular Sequence Data
  • Peptides
  • Protein Binding
  • Protein Kinase C
  • Receptors, Cell Surface
  • Signal Transduction
  • Tetradecanoylphorbol Acetate