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A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae.

The PKC1-MPK1 pathway in yeast functions in the maintenance of cell wall integrity and in the stress response. We have identified a family of genes that are putative regulators of this pathway. WSC1, WSC2, and WSC3 encode predicted integral membrane proteins with a conserved cysteine motif and a WSC1-green fluorescence protein fusion protein localizes to the plasma membrane. Deletion of WSC results in phenotypes similar to mutants in the PKC1-MPK1 pathway and an increase in the activity of MPK1 upon a mild heat treatment is impaired in a wscDelta mutant. Genetic analysis places the function of WSC upstream of PKC1, suggesting that they play a role in its activation. We also find a genetic interaction between WSC and the RAS-cAMP pathway. The RAS-cAMP pathway is required for cell cycle progression and for the heat shock response. Overexpression of WSC suppresses the heat shock sensitivity of a strain in which RAS is hyperactivated and the heat shock sensitivity of a wscDelta strain is rescued by deletion of RAS2. The functional characteristics and cellular localization of WSC suggest that they may mediate intracellular responses to environmental stress in yeast.

Pubmed ID: 9391108


  • Verna J
  • Lodder A
  • Lee K
  • Vagts A
  • Ballester R


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

December 9, 1997

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Cell Wall
  • Cyclic AMP
  • Fungal Proteins
  • Gene Deletion
  • Genes, Fungal
  • Genes, ras
  • Hot Temperature
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Mitogen-Activated Protein Kinases
  • Molecular Sequence Data
  • Multigene Family
  • Mutation
  • Phenotype
  • Protein Kinase C
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid