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Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells.

Cell | Nov 14, 1997

http://www.ncbi.nlm.nih.gov/pubmed/9390559

Members of the Tc1/mariner superfamily of transposons isolated from fish appear to be transpositionally inactive due to the accumulation of mutations. Molecular phylogenetic data were used to construct a synthetic transposon, Sleeping Beauty, which could be identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase binds to the inverted repeats of salmonid transposons in a substrate-specific manner, and it mediates precise cut-and-paste transposition in fish as well as in mouse and human cells. Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis.

Pubmed ID: 9390559 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Carps | Cell Line | DNA | DNA Transposable Elements | DNA, Recombinant | Enzyme Activation | Humans | Mice | Molecular Sequence Data | Recombinant Fusion Proteins | Salmonidae | Transfection | Transposases

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Associated grants

  • Agency: NCRR NIH HHS, Id: R01-RR06625

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