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Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.

A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59 and spU2AF23, from fission yeast. We now report a novel U2AF-associated-protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal.

Pubmed ID: 9371883

Authors

  • McKinney R
  • Wentz-Hunter K
  • Schmidt H
  • Potashkin J

Journal

Current genetics

Publication Data

November 4, 1997

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01GM47487

Mesh Terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • Genes, Fungal
  • Molecular Sequence Data
  • Nuclear Proteins
  • RNA Splicing
  • RNA, Fungal
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Saccharomyces cerevisiae
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Homology, Amino Acid