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Diabetes, defective pancreatic morphogenesis, and abnormal enteroendocrine differentiation in BETA2/neuroD-deficient mice.

Genes & development | Sep 15, 1997

Candidate transcription factors involved in pancreatic endocrine development have been isolated using insulin gene regulation as a paradigm. The cell-type restricted basic helix-loop-helix (bHLH) gene, BETA2/NeuroD, expressed in pancreatic endocrine cells, the intestine, and the brain, activates insulin gene transcription and can induce neurons to differentiate. To understand the importance of BETA2 in pancreatic endocrine cell differentiation, mice lacking a functional BETA2 gene were generated by gene targeting experiments. Mice carrying a targeted disruption of the BETA2 gene developed severe diabetes and died perinatally. Homozygous BETA2 null mice had a striking reduction in the number of insulin-producing beta cells and failed to develop mature islets. Islet morphogenesis appeared to be arrested between E14.5 and E17.5, a period characterized by major expansion of the beta cell population. The presence of severe diabetes in these mice suggests that proper islet structure plays an important role in blood glucose homeostasis. In addition, secretin- and cholecystokinin-producing enteroendocrine cells failed to develop in the absence of BETA2. The absence of these two pancreatic secretagogs may explain the abnormal cellular polarity and inability to secrete zymogen granules in pancreatic acinar exocrine cells. The nervous system appeared to develop normally, despite abundant expression of BETA2 in differentiating neurons. Thus, BETA2 is critical for the normal development of several specialized cell types arising from the gut endoderm.

Pubmed ID: 9308961 RIS Download

Mesh terms: Animals | Basic Helix-Loop-Helix Transcription Factors | Cholecystokinin | DNA-Binding Proteins | Diabetes Mellitus, Experimental | Gene Targeting | Helix-Loop-Helix Motifs | Mice | Mice, Mutant Strains | Morphogenesis | Pancreas | Secretin | Trans-Activators

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Associated grants

  • Agency: NICHD NIH HHS, Id: R37 HD017379
  • Agency: NICHD NIH HHS, Id: HD17379
  • Agency: NIDDK NIH HHS, Id: P30 DK034928
  • Agency: NIDDK NIH HHS, Id: DK34928
  • Agency: NICHD NIH HHS, Id: R01 HD017379
  • Agency: NIDDK NIH HHS, Id: DK43673
  • Agency: NIDDK NIH HHS, Id: R01 DK043673

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