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BAG-1 modulates the chaperone activity of Hsp70/Hsc70.

The 70 kDa heat shock family of molecular chaperones is essential to a variety of cellular processes, yet it is unclear how these proteins are regulated in vivo. We present evidence that the protein BAG-1 is a potential modulator of the molecular chaperones, Hsp70 and Hsc70. BAG-1 binds to the ATPase domain of Hsp70 and Hsc70, without requirement for their carboxy-terminal peptide-binding domain, and can be co-immunoprecipitated with Hsp/Hsc70 from cell lysates. Purified BAG-1 and Hsp/Hsc70 efficiently form heteromeric complexes in vitro. BAG-1 inhibits Hsp/Hsc70-mediated in vitro refolding of an unfolded protein substrate, whereas BAG-1 mutants that fail to bind Hsp/Hsc70 do not affect chaperone activity. The binding of BAG-1 to one of its known cellular targets, Bcl-2, in cell lysates was found to be dependent on ATP, consistent with the possible involvement of Hsp/Hsc70 in complex formation. Overexpression of BAG-1 also protected certain cell lines from heat shock-induced cell death. The identification of Hsp/Hsc70 as a partner protein for BAG-1 may explain the diverse interactions observed between BAG-1 and several other proteins, including Raf-1, steroid hormone receptors and certain tyrosine kinase growth factor receptors. The inhibitory effects of BAG-1 on Hsp/Hsc70 chaperone activity suggest that BAG-1 represents a novel type of chaperone regulatory proteins and thus suggest a link between cell signaling, cell death and the stress response.

Pubmed ID: 9305631


  • Takayama S
  • Bimston DN
  • Matsuzawa S
  • Freeman BC
  • Aime-Sempe C
  • Xie Z
  • Morimoto RI
  • Reed JC


The EMBO journal

Publication Data

August 15, 1997

Associated Grants

  • Agency: NCI NIH HHS, Id: CA-67329
  • Agency: NIGMS NIH HHS, Id: GM-38109

Mesh Terms

  • Adenosine Triphosphatases
  • Adenosine Triphosphate
  • Brain
  • Carrier Proteins
  • Cell Death
  • Cell Line
  • Chromatography, Gel
  • Cloning, Molecular
  • DNA-Binding Proteins
  • HSC70 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins
  • Humans
  • Immunoblotting
  • Oligopeptides
  • Peptides
  • Protein Binding
  • Protein Conformation
  • Protein Denaturation
  • Protein Folding
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Transfection
  • beta-Galactosidase