Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95.

BACKGROUND: The human homologue of the Drosophila discs large tumour suppressor protein (hDLG) and closely related proteins such as postsynaptic density protein 95 kDa (PSD-95) are associated with N-methyl-D-aspartate receptors (NMDA-R) and Shaker-type K+ channels, and are thought to be involved in their clustering. RESULTS: We have identified a protein named DAP-1 that binds to the guanylate kinase-like domains of hDLG and PSD-95. DAP-1 was found to associate with hDLG, PSD-95, NMDA-R and adenomatous polyposis coli protein (APC). Furthermore, we found that DAP-1 is specifically expressed in the brain and colocalizes with PSD-95 and APC in mouse cerebellum. We also found that DAP-1 is colocalized with PSD-95 and NMDA-R at the synapses in cultured rat hippocampal neurons. CONCLUSION: Our findings suggest that DAP-1 may play several roles in the molecular organization of synapses and neuronal cell signalling by interacting with hDLG and PSD-95, which in turn are associated with receptors, ion channels and APC.

Pubmed ID: 9286858


  • Satoh K
  • Yanai H
  • Senda T
  • Kohu K
  • Nakamura T
  • Okumura N
  • Matsumine A
  • Kobayashi S
  • Toyoshima K
  • Akiyama T


Genes to cells : devoted to molecular & cellular mechanisms

Publication Data

June 23, 1997

Associated Grants


Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Adenomatous Polyposis Coli Protein
  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins
  • Binding Sites
  • Brain
  • Cerebellum
  • Cloning, Molecular
  • Cytoskeletal Proteins
  • Guanylate Kinase
  • Hippocampus
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Membrane Proteins
  • Mice
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Neurons
  • Nucleoside-Phosphate Kinase
  • Precipitin Tests
  • Proteins
  • Rabbits
  • Rats
  • Receptors, N-Methyl-D-Aspartate
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Amino Acid