• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95.

BACKGROUND: The human homologue of the Drosophila discs large tumour suppressor protein (hDLG) and closely related proteins such as postsynaptic density protein 95 kDa (PSD-95) are associated with N-methyl-D-aspartate receptors (NMDA-R) and Shaker-type K+ channels, and are thought to be involved in their clustering. RESULTS: We have identified a protein named DAP-1 that binds to the guanylate kinase-like domains of hDLG and PSD-95. DAP-1 was found to associate with hDLG, PSD-95, NMDA-R and adenomatous polyposis coli protein (APC). Furthermore, we found that DAP-1 is specifically expressed in the brain and colocalizes with PSD-95 and APC in mouse cerebellum. We also found that DAP-1 is colocalized with PSD-95 and NMDA-R at the synapses in cultured rat hippocampal neurons. CONCLUSION: Our findings suggest that DAP-1 may play several roles in the molecular organization of synapses and neuronal cell signalling by interacting with hDLG and PSD-95, which in turn are associated with receptors, ion channels and APC.

Pubmed ID: 9286858


  • Satoh K
  • Yanai H
  • Senda T
  • Kohu K
  • Nakamura T
  • Okumura N
  • Matsumine A
  • Kobayashi S
  • Toyoshima K
  • Akiyama T


Genes to cells : devoted to molecular & cellular mechanisms

Publication Data

June 23, 1997

Associated Grants


Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Adenomatous Polyposis Coli Protein
  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins
  • Binding Sites
  • Brain
  • Cerebellum
  • Cloning, Molecular
  • Cytoskeletal Proteins
  • Guanylate Kinase
  • Hippocampus
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Membrane Proteins
  • Mice
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Neurons
  • Nucleoside-Phosphate Kinase
  • Precipitin Tests
  • Proteins
  • Rabbits
  • Rats
  • Receptors, N-Methyl-D-Aspartate
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Amino Acid