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14-3-3 proteins are essential for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae.

14-3-3 proteins are highly conserved ubiquitous proteins whose explicit functions have remained elusive. Here, we show that the S. cerevisiae 14-3-3 homologs BMH1 and BMH2 are not essential for viability or mating MAPK cascade signaling, but they are essential for pseudohyphal-development MAPK cascade signaling and other processes. Activated alleles of RAS2 and CDC42 induce pseudohyphal development and FG(TyA)-lacZ signaling in Bmh+ strains but not in ste20 (p65PAK) or bmh1 bmh2 mutant strains. Moreover, Bmh1p and Bmh2p associate with Ste20p in vivo. Three alleles of BMH1 encode proteins defective for FG(TyA)-lacZ signaling and association with Ste20p, yet these alleles complement other 14-3-3 functions. Therefore, the 14-3-3 proteins are specifically required for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae.

Pubmed ID: 9215628

Authors

  • Roberts RL
  • Mösch HU
  • Fink GR

Journal

Cell

Publication Data

June 27, 1997

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI07348
  • Agency: NIGMS NIH HHS, Id: GM40266

Mesh Terms

  • 14-3-3 Proteins
  • Adaptation, Physiological
  • Alleles
  • Biological Evolution
  • Cell Survival
  • Fungal Proteins
  • GTP-Binding Proteins
  • Glycogen
  • Intracellular Signaling Peptides and Proteins
  • Lac Operon
  • MAP Kinase Kinase Kinases
  • Molecular Sequence Data
  • Mutagenesis
  • Phenotype
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases
  • Recombinant Proteins
  • Reproduction
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Species Specificity
  • ras Proteins