Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

14-3-3 proteins are essential for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae.

Cell | Jun 27, 1997

http://www.ncbi.nlm.nih.gov/pubmed/9215628

14-3-3 proteins are highly conserved ubiquitous proteins whose explicit functions have remained elusive. Here, we show that the S. cerevisiae 14-3-3 homologs BMH1 and BMH2 are not essential for viability or mating MAPK cascade signaling, but they are essential for pseudohyphal-development MAPK cascade signaling and other processes. Activated alleles of RAS2 and CDC42 induce pseudohyphal development and FG(TyA)-lacZ signaling in Bmh+ strains but not in ste20 (p65PAK) or bmh1 bmh2 mutant strains. Moreover, Bmh1p and Bmh2p associate with Ste20p in vivo. Three alleles of BMH1 encode proteins defective for FG(TyA)-lacZ signaling and association with Ste20p, yet these alleles complement other 14-3-3 functions. Therefore, the 14-3-3 proteins are specifically required for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae.

Pubmed ID: 9215628 RIS Download

Mesh terms: 14-3-3 Proteins | Adaptation, Physiological | Alleles | Biological Evolution | Cell Survival | Fungal Proteins | GTP-Binding Proteins | Glycogen | Intracellular Signaling Peptides and Proteins | Lac Operon | MAP Kinase Kinase Kinases | Molecular Sequence Data | Mutagenesis | Phenotype | Protein Structure, Tertiary | Protein-Serine-Threonine Kinases | Recombinant Proteins | Reproduction | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Sequence Homology, Amino Acid | Signal Transduction | Species Specificity | ras Proteins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIAID NIH HHS, Id: AI07348
  • Agency: NIGMS NIH HHS, Id: GM40266

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.