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Homo- and heterodimeric interactions between the gene products of PKD1 and PKD2.

PKD1 and PKD2 are two recently identified genes that are responsible for the vast majority of autosomal polycystic kidney disease, a common inherited disease that causes progressive renal failure. PKD1 encodes polycystin, a large glycoprotein that contains several extracellular motifs indicative of a role in cell-cell or cell-matrix interactions, and the PKD2 encodes a protein with homology to a voltage-activated calcium channel and to PKD1. It is currently unknown how mutations of either protein functionally cause autosomal polycystic kidney disease. We show that PKD1 and PKD2 interact through their C-terminal cytoplasmic tails. This interaction resulted in an up-regulation of PKD1 but not PKD2. Furthermore, the cytoplasmic tail of PKD2 but not PKD1 formed homodimers through a coiled-coil domain distinct from the region required for interaction with PKD1. These interactions suggest that PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis and that PKD1 may require the presence of PKD2 for stable expression.

Pubmed ID: 9192675

Authors

  • Tsiokas L
  • Kim E
  • Arnould T
  • Sukhatme VP
  • Walz G

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

June 24, 1997

Associated Grants

  • Agency: NIMH NIH HHS, Id: MH-01147
  • Agency: NIDDK NIH HHS, Id: R01-DK-51060

Mesh Terms

  • Dimerization
  • Humans
  • Membrane Proteins
  • Protein Binding
  • Proteins
  • Recombinant Proteins
  • Signal Transduction
  • TRPP Cation Channels