Literature search services are currently unavailable. During our hosting provider's UPS upgrade we experienced a hardware failure and are currently working to resolve the issue.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A membrane cofactor protein transgenic mouse model for the study of discordant xenograft rejection.

BACKGROUND: In recent years, interest has been revived in the possibility of transplanting organs into humans from a phylogenetically disparate species such as the pig (xenotransplantation). Such discordant xenografts, however, are subject to hyperacute rejection (HAR) and activation of host complement plays a major role in this rejection. This problem may be solved through the use of transgenic technology by providing the grafted tissue with molecules that down-regulate the action of host complement. RESULTS: Transgenesis with a yeast artificial chromosome (YAC) was used to produce transgenic mice with the complete genomic gene of the human complement regulator membrane cofactor protein (MCP). Transgenic mice were obtained that exhibit full regulation of MCP as normally observed in humans. Hearts from these mice were shown to be significantly protected from HAR caused by human serum in an in vivo experimental procedure. CONCLUSIONS: We conclude that MCP can protect discordant xenografts from HAR caused by human serum and that transgenic mice can be used effectively as in vivo models for the study of the role of human complement regulatory molecules in xenotransplantation.

Pubmed ID: 9135084


  • Yannoutsos N
  • Ijzermans JN
  • Harkes C
  • Bonthuis F
  • Zhou CY
  • White D
  • Marquet RL
  • Grosveld F


Genes to cells : devoted to molecular & cellular mechanisms

Publication Data

April 23, 1996

Associated Grants


Mesh Terms

  • Animals
  • Antigens, CD
  • Antigens, CD46
  • Blotting, Northern
  • Chromosome Mapping
  • Chromosomes, Artificial, Yeast
  • Cloning, Molecular
  • Complement Inactivator Proteins
  • Disease Models, Animal
  • Gene Expression
  • Genes, Regulator
  • Graft Rejection
  • Heart Transplantation
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Membrane Glycoproteins
  • Mice
  • Mice, Transgenic
  • RNA
  • Transplantation, Heterologous