Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells.

Genes & development | Jan 1, 1997

http://www.ncbi.nlm.nih.gov/pubmed/9000051

Here we describe the cloning and characterization of a PAS domain transcription factor termed endothelial PAS-1 (EPAS1). This protein shares 48% sequence identity with hypoxia inducible factor (HIF-1alpha) and lesser similarity with other members of the basic helix-loop-helix/PAS domain family of transcription factors. Like HIF-1alpha, EPAS1 binds to and activates transcription from a DNA element originally isolated from the erythropoietin gene and containing the sequence 5'-GCCCTACGTGCTGTCTCA-3'. Activation by both HIF-1alpha and EPAS1 is stimulated by hypoxic conditions. EPAS1 forms a heterodimeric complex with the aryl hydrocarbon nuclear transporter prior to transcriptional activation of target genes. EPAS1 expression is limited to the endothelium of mouse embryos and, in agreement with its cell type-specific expression pattern, is capable of specifically activating the transcription of the endothelial tyrosine kinase gene Tie-2. These observations raise the possibility that EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia.

Pubmed ID: 9000051 RIS Download

Mesh terms: Amino Acid Sequence | Aryl Hydrocarbon Receptor Nuclear Translocator | Blotting, Northern | Cloning, Molecular | DNA-Binding Proteins | Dimerization | Genes, Reporter | HeLa Cells | Helix-Loop-Helix Motifs | Humans | Hypoxia-Inducible Factor 1 | Hypoxia-Inducible Factor 1, alpha Subunit | In Situ Hybridization | Molecular Sequence Data | Nuclear Proteins | RNA | Receptors, Aryl Hydrocarbon | Sequence Alignment | Sequence Analysis | Transcription Factors | Transcriptional Activation | Transfection

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA52121
  • Agency: NIDDK NIH HHS, Id: DK-47657

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.