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Subregion- and cell type-restricted gene knockout in mouse brain.

Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

Pubmed ID: 8980237


  • Tsien JZ
  • Chen DF
  • Gerber D
  • Tom C
  • Mercer EH
  • Anderson DJ
  • Mayford M
  • Kandel ER
  • Tonegawa S



Publication Data

December 27, 1996

Associated Grants

  • Agency: NINDS NIH HHS, Id: NS32925

Mesh Terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Gene Expression
  • Gene Expression Regulation
  • Genetic Engineering
  • Gestational Age
  • Hippocampus
  • Integrases
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Promoter Regions, Genetic
  • RNA, Messenger
  • Recombination, Genetic
  • Sequence Deletion
  • Viral Proteins