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Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system.

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.

Pubmed ID: 8921390


  • Shen Z
  • Pardington-Purtymun PE
  • Comeaux JC
  • Moyzis RK
  • Chen DJ



Publication Data

October 15, 1996

Associated Grants

  • Agency: NCI NIH HHS, Id: CA50519

Mesh Terms

  • Carrier Proteins
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16
  • DNA Repair
  • DNA-Binding Proteins
  • Fungal Proteins
  • Humans
  • Ligases
  • Male
  • Molecular Sequence Data
  • Protein Binding
  • RNA, Messenger
  • Rad51 Recombinase
  • Rad52 DNA Repair and Recombination Protein
  • SUMO-1 Protein
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Testis
  • Tumor Suppressor Protein p53
  • Ubiquitin-Conjugating Enzymes