Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system.
The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.
Pubmed ID: 8921390 RIS Download
Carrier Proteins | Chromosome Mapping | Chromosomes, Human, Pair 16 | DNA Repair | DNA-Binding Proteins | Fungal Proteins | Humans | Ligases | Male | Molecular Sequence Data | Protein Binding | RNA, Messenger | Rad51 Recombinase | Rad52 DNA Repair and Recombination Protein | SUMO-1 Protein | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Schizosaccharomyces pombe Proteins | Testis | Tumor Suppressor Protein p53 | Ubiquitin-Conjugating Enzymes