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hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II.

TFIID is the main sequence-specific DNA-binding component of the RNA polymerase II (Pol II) transcriptional machinery. It is a multiprotein complex composed of the TATA-binding protein (TBP) and TBP-associated factors (TAF(II)s). Here we report the cloning and characterization of a novel human TBP-associated factor, hTAF(II)68. It contains a consensus RNA-binding domain (RNP-CS) and binds not only RNA, but also single stranded (ss) DNA. hTAF(II)68 shares extensive sequence similarity with TLS/FUS and EWS, two human nuclear RNA-binding pro-oncoproteins which are products of genes commonly translocated in human sarcomas. Like hTAF(II)68, TLS/FUS is also associated with a sub-population of TFIID complexes chromatographically separable from those containing hTAF(II)68. Therefore, these RNA and/or ssDNA-binding proteins may play specific roles during transcription initiation at distinct promoters. Moreover, we demonstrate that hTAF(II)68 co-purifies also with the human RNA polymerase II and can enter the preinitiation complex together with Pol II.

Pubmed ID: 8890175


  • Bertolotti A
  • Lutz Y
  • Heard DJ
  • Chambon P
  • Tora L


The EMBO journal

Publication Data

September 16, 1996

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Polymerase II
  • DNA, Single-Stranded
  • Electrophoresis, Polyacrylamide Gel
  • HeLa Cells
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Humans
  • Molecular Sequence Data
  • Neoplasm Proteins
  • RNA
  • RNA, Heterogeneous Nuclear
  • RNA-Binding Protein EWS
  • RNA-Binding Protein FUS
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Sequence Alignment
  • TATA-Binding Protein Associated Factors
  • Transcription Factor TFIID
  • Transcription Factors