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The metastasis suppressor candidate nucleotide diphosphate kinase NM23 specifically interacts with members of the ROR/RZR nuclear orphan receptor subfamily.

We have cloned proteins that interact with the nuclear orphan receptor RZR beta using the yeast two-hybrid system. We identified, amongst a number of other genes, the nucleoside diphosphate kinase (NDPK)-2 also known as Nm23-2, c-myc regulatory factor PuF and differentiation inhibitory factor, RZR beta specifically interacts with Nm23-2 but not with the closely related tumor metastasis suppressor candidate gene product Nm23-1. In contrast ROR alpha interacts with both Nm23 proteins. These findings were corroborated by in vitro interaction assays based on GST-pulldown experiments. With-n-myc we propose a candidate gene regulated by ROR alpha/RZR beta and Nm23, based on the finding that the respective DNA binding sites in the first intron are conserved in several mammalian species.

Pubmed ID: 8858107


  • Paravicini G
  • Steinmayr M
  • AndrĂ© E
  • Becker-AndrĂ© M


Biochemical and biophysical research communications

Publication Data

October 3, 1996

Associated Grants


Mesh Terms

  • Animals
  • Base Sequence
  • Genes, myc
  • Humans
  • Mice
  • Molecular Sequence Data
  • Monomeric GTP-Binding Proteins
  • NM23 Nucleoside Diphosphate Kinases
  • Nucleoside-Diphosphate Kinase
  • Protein Binding
  • Rats
  • Receptors, Cytoplasmic and Nuclear
  • Sequence Homology, Nucleic Acid
  • Substrate Specificity
  • Transcription Factors