• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae.

The Rho-type GTPase Cdc42p is required for cell polarization and bud emergence in Saccharomyces cerevisiae. To identify genes whose functions are linked to CDC42, we screened for (i) multicopy suppressors of a Ts- cdc42 mutant, (ii) mutants that require multiple copies of CDC42 for survival, and (iii) mutations that display synthetic lethality with a partial-loss-of-function allele of CDC24, which encodes a guanine nucleotide exchange factor for Cdc42p. In all three screens, we identified a new gene, BEM4. Cells from which BEM4 was deleted were inviable at 37 degrees C. These cells became unbudded, large, and round, consistent with a model in which Bem4p acts together with Cdc42p in polarity establishment and bud emergence. In some strains, the ability of CDC42 to serve as a multicopy suppressor of the Ts- growth defect of deltabem4 cells required co-overexpression of Rho1p, which is an essential Rho-type GTPase necessary for cell wall integrity. This finding suggests that Bem4p also affects Rho1p function. Bem4p displayed two-hybrid interactions with Cdc42p, Rho1p, and two of the three other known yeast Rho-type GTPases, suggesting that Bem4p can interact with multiple Rho-type GTPases. Models for the role of Bem4p include that it serves as a chaperone or modulates the interaction of these GTPases with one or more of their targets or regulators.

Pubmed ID: 8754839


  • Mack D
  • Nishimura K
  • Dennehey BK
  • Arbogast T
  • Parkinson J
  • Toh-e A
  • Pringle JR
  • Bender A
  • Matsui Y


Molecular and cellular biology

Publication Data

August 20, 1996

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM12276
  • Agency: NIGMS NIH HHS, Id: GM31006
  • Agency: NIGMS NIH HHS, Id: GM46271

Mesh Terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • GTP-Binding Proteins
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Genes, Suppressor
  • Guanine Nucleotide Exchange Factors
  • Intracellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Protein Binding
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Restriction Mapping
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
  • rho GTP-Binding Proteins