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Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.


Using the yeast two-hybrid system, we have identified a human ubiquitin-conjugating enzyme (hE2-25K) as a protein that interacts with the gene product for Huntington disease (HD) (Huntingtin). This protein has complete amino acid identity with the bovine E2-25K protein and has striking similarity to the UBC-1, -4 and -5 enzymes of Saccharomyces cerevisiae. This protein is highly expressed in brain and a slightly larger protein recognized by an anti-E2-25K polyclonal antibody is selectively expressed in brain regions affected in HD. The huntingtin-E2-25K interaction is not obviously modulated by CAG length. We also demonstrate that huntingtin is ubiquitinated. These findings have implications for the regulated catabolism of the gene product for HD.

Pubmed ID: 8702625


  • Kalchman MA
  • Graham RK
  • Xia G
  • Koide HB
  • Hodgson JG
  • Graham KC
  • Goldberg YP
  • Gietz RD
  • Pickart CM
  • Hayden MR


The Journal of biological chemistry

Publication Data

August 9, 1996

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain
  • Cattle
  • Chromosome Mapping
  • Chromosomes, Human, Pair 4
  • DNA, Complementary
  • Humans
  • Ligases
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitins