• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


mRNA profiling of rat islet tumors reveals nkx 6.1 as a beta-cell-specific homeodomain transcription factor.

Development of a high capacity multiplex reverse transcriptase-polymerase chain reaction protocol has allowed us to screen lineage related rat islet tumors classified as alpha-, beta-, and delta-like as judged by their hormone profile for differential expression of more than 50 selected genes. We find that in addition to insulin the insulinoma express the normal beta-cell markers Pdx-1, IAPP, and Glut-2, and that these markers are absent from the glucagonoma: a reflection of the normal alpha-cell. Furthermore, this study suggests that the GLP-1, glucagon, GIP, IGF-1, and insulin receptors as well as E-cadherin, R-cadherin, Id-1, and Id-2 are differentially expressed within the islet of Langerhans. Importantly, insulinoma-specific expression of the recently cloned homeodomain protein Nkx 6.1 predicted beta-cell-specific expression in the normal islet. Immunohistochemistry using antibodies raised against recombinant Nkx 6.1 did indeed localize Nkx 6.1 expression exclusively to the nuclei of normal islet beta-cells. Apart from pancreatic islets only the antral part of the stomach contained Nkx 6.1 mRNA. We conclude that multiplex reverse transcriptase-polymerase chain reaction-based mRNA profiling is a powerful tool to identify differentially expressed genes within phenotypically related cells and propose that Nkx 6.1 is involved in specifying the unique characteristics of the beta-cell.

Pubmed ID: 8702531


  • Jensen J
  • Serup P
  • Karlsen C
  • Nielsen TF
  • Madsen OD


The Journal of biological chemistry

Publication Data

August 2, 1996

Associated Grants


Mesh Terms

  • Adenoma, Islet Cell
  • Animals
  • Base Sequence
  • Biological Markers
  • DNA Primers
  • DNA, Complementary
  • Gene Expression
  • Hexokinase
  • Homeodomain Proteins
  • Immunohistochemistry
  • Islets of Langerhans
  • Molecular Sequence Data
  • Monosaccharide Transport Proteins
  • Pancreatic Hormones
  • Pancreatic Neoplasms
  • Phenotype
  • Polymerase Chain Reaction
  • RNA, Messenger
  • RNA, Neoplasm
  • Rats
  • Receptors, Cell Surface
  • Transcription Factors