Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Targeted disruption of the mouse beta1-adrenergic receptor gene: developmental and cardiovascular effects.

http://www.ncbi.nlm.nih.gov/pubmed/8693001

At least three distinct beta-adrenergic receptor (beta-AR) subtypes exist in mammals. These receptors modulate a wide variety of processes, from development and behavior, to cardiac function, metabolism, and smooth muscle tone. To understand the roles that individual beta-AR subtypes play in these processes, we have used the technique of gene targeting to create homozygous beta 1-AR null mutants (beta 1-AR -/-) in mice. The majority of beta 1-AR -/- mice die prenatally, and the penetrance of lethality shows strain dependence. Beta l-AR -/- mice that do survive to adulthood appear normal, but lack the chronotropic and inotropic responses seen in wild-type mice when beta-AR agonists such as isoproterenol are administered. Moreover, this lack of responsiveness is accompanied by markedly reduced stimulation of adenylate cyclase in cardiac membranes from beta 1-AR -/- mice. These findings occur despite persistent cardiac beta 2-AR expression, demonstrating the importance of beta 1-ARs for proper mouse development and cardiac function, while highlighting functional differences between beta-AR subtypes.

Pubmed ID: 8693001 RIS Download

Mesh terms: Adenylate Cyclase | Adrenergic beta-Agonists | Adrenergic beta-Antagonists | Aging | Animals | Cell Membrane | Chimera | Crosses, Genetic | Death | Female | Gene Expression | Heart | Heart Rate | Heart Ventricles | Homozygote | Imidazoles | Isoproterenol | Kinetics | Lung | Male | Methoxyhydroxyphenylglycol | Mice | Mice, Inbred C57BL | Mice, Inbred DBA | Mice, Knockout | Myocardial Contraction | Myocardium | Norepinephrine | Receptors, Adrenergic, beta-1 | Restriction Mapping | Stem Cells

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.