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Identification of a novel syntaxin- and synaptobrevin/VAMP-binding protein, SNAP-23, expressed in non-neuronal tissues.

The specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP-25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are ubiquitously expressed proteins and are believed to be involved in vesicular transport in most (if not all) cells. However, SNAP-25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP-25 exists to facilitate transport vesicle/target membrane fusion in other tissues. Using the yeast two-hybrid system, we have identified a 23-kDa protein from human B lymphocytes (termed SNAP-23) that binds tightly to multiple syntaxins and synaptobrevins/VAMPs in vitro. SNAP-23 is 59% identical with SNAP-25. Unlike SNAP-25, SNAP-23 was expressed in all tissues examined. These findings suggest that SNAP-23 is an essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion in all mammalian cells.

Pubmed ID: 8663154


  • Ravichandran V
  • Chawla A
  • Roche PA


The Journal of biological chemistry

Publication Data

June 7, 1996

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression
  • Humans
  • In Vitro Techniques
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Protein Binding
  • Qa-SNARE Proteins
  • Qb-SNARE Proteins
  • Qc-SNARE Proteins
  • R-SNARE Proteins
  • Rats
  • Recombinant Fusion Proteins
  • Sequence Homology, Amino Acid
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Synaptosomal-Associated Protein 25
  • Vesicular Transport Proteins