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The mammalian immediate-early TIS21 protein and the leukemia-associated BTG1 protein interact with a protein-arginine N-methyltransferase.

The TIS21 immediate-early gene and leukemia-associated BTG1 gene encode proteins with similar sequences. Two-hybrid analysis identified a protein that interacts with TIS21 and BTG1. Sequence motifs associated with S-adenosyl-L-methionine binding suggested this protein might have methyltransferase activity. A glutathione S-transferase (GST) fusion of the putative methyltransferase modifies arginine residues, in appropriate protein substrates, to form NG-monomethyl and NG,NG-dimethylarginine (asymmetric). We term the protein- arginine N-methyltransferase (EC gene "PRMT1, " for protein-arginine methyltransferase 1. GST-TIS21 and GST-BTG1 fusion proteins qualitatively and quantitatively modulate endogenous PRMT1 activity, using control and hypomethylated RAT1 cell extracts as methyl-accepting substrates. PRMT1 message appears ubiquitous, and is constitutive in mitogen-stimulated cells. Modulation of PRMT1 activity by transiently expressed regulatory subunits may be an additional mode of signal transduction following ligand stimulation.

Pubmed ID: 8663146 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Base Sequence | Cloning, Molecular | DNA Primers | DNA, Complementary | Escherichia coli | Genes, Tumor Suppressor | Glutathione Transferase | Humans | Immediate-Early Proteins | Intracellular Signaling Peptides and Proteins | Leukemia, Lymphocytic, Chronic, B-Cell | Macromolecular Substances | Mammals | Methyltransferases | Molecular Sequence Data | Neoplasm Proteins | Open Reading Frames | Polymerase Chain Reaction | Protein Biosynthesis | Protein Structure, Secondary | Protein-Arginine N-Methyltransferases | Proteins | Rats | Recombinant Fusion Proteins | Saccharomyces cerevisiae | Sequence Homology, Amino Acid | Tumor Suppressor Proteins

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM24797
  • Agency: NIGMS NIH HHS, Id: GM26020

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