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Cloning and functional expression of mCCR2, a murine receptor for the C-C chemokines JE and FIC.

The C-C chemokines human monocyte chemoattractant protein-1 and -3 (MCP-1 and MCP-3) and mouse JE and FIC are potent activators of monocytes. Several receptors for MCP-1 and MCP-3 have been cloned from human monocytic cell lines, and one of these receptors, CCR2B, binds both MCP-l and MCP-3. Thus far, no murine receptors for JE or FIC have been reported. We have cloned a novel murine C-C chemokine receptor, designated mouse CCR2 (mCCR2), from the mouse monocyte cell line WEHI265.1. The predicted 373-amino acid sequence of mCCR2 shows highest identity (80%) with CCR2B. When stably expressed in human embryonic kidney 293 cells, mCCR2 specifically bound 125I-JE with high affinity. FIC was less potent than JE in competing 125I-JE binding to mCCR2-expressing cells, while three other mouse chemokines, MIP-1alpha, C10, and N51/KC, did not compete. mccr2 mRNA expression was detected in elicited peritoneal macrophages as well as in several mouse organs. The cloning of mCCR2 provides an important tool to investigate monocyte/macrophage responses to JE and FIC, to identify other targets for their action, and potentially to study models of CCR2 function in the mouse.

Pubmed ID: 8662823


  • Kurihara T
  • Bravo R


The Journal of biological chemistry

Publication Data

May 17, 1996

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chemokine CCL2
  • Chemokine CCL7
  • Cloning, Molecular
  • Cytokines
  • Humans
  • Immediate-Early Proteins
  • Mice
  • Molecular Sequence Data
  • Monocyte Chemoattractant Proteins
  • RNA, Messenger
  • Receptors, CCR2
  • Receptors, Chemokine
  • Receptors, Cytokine