CC CKR5: a RANTES, MIP-1alpha, MIP-1beta receptor as a fusion cofactor for macrophage-tropic HIV-1.
Human immunodeficiency virus-type 1 (HIV-1) entry requires fusion cofactors on the CD4+ target cell. Fusin, a heterotrimeric GTP-binding protein (G protein)-coupled receptor, serves as a cofactor for T cell line-tropic isolates. The chemokines RANTES, MIP-1alpha, and MIP-1beta, which suppress infection by macrophage-tropic isolates, selectively inhibited cell fusion mediated by the corresponding envelope glycoproteins (Envs). Recombinant CC CKR5, a G protein-coupled receptor for these chemokines, rendered CD4-expressing nonhuman cells fusion-competent preferentially with macrophage-tropic Envs. CC CKR5 messenger RNA was detected selectively in cell types susceptible to macrophage-tropic isolates. CC CKR5 is thus a fusion cofactor for macrophage-tropic HIV-1 strains.
Pubmed ID: 8658171 RIS Download
3T3 Cells | Animals | Antigens, CD4 | Cell Fusion | Chemokine CCL3 | Chemokine CCL4 | Chemokine CCL5 | Chemokines | Gene Products, env | Giant Cells | HIV-1 | HeLa Cells | Humans | Macrophage Inflammatory Proteins | Macrophages | Membrane Fusion | Mice | Monokines | Receptors, CCR5 | Receptors, Cytokine | Receptors, HIV | Recombinant Proteins | T-Lymphocytes | Tumor Cells, Cultured