Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Cloning and characterization of a specific coactivator, ARA70, for the androgen receptor in human prostate cells.

http://www.ncbi.nlm.nih.gov/pubmed/8643607

The androgen receptor (AR) is a member of the steroid receptor superfamily that plays an important role in male sexual differentiation and prostate cell proliferation. Mutations or abnormal expression of AR in prostate cancer can play a key role in the process that changes prostate cancer from androgen-dependent to an androgen-independent stage. Using a yeast two-hybrid system, we were able to isolate a ligand-dependent AR-associated protein (ARA70), which functions as an activator to enhance AR transcriptional activity 10-fold in the presence of 10(-10) M dihydrotestosterone or 10(-9) M testosterone, but not 10(-6) M hydroxyflutamide in human prostate cancer DU145 cells. Our data further indicated that ARA70 Will only slightly induce the transcriptional activity of other steroid receptors such as estrogen receptor, glucocorticoid receptor, and progesterone receptor in DU145 cells. Together, these data suggest that AR may need a specific coactivator(s) such as ARA70 for optimal androgen activity.

Pubmed ID: 8643607 RIS Download

Mesh terms: Amino Acid Sequence | Base Sequence | Blotting, Northern | Cell Line | Chloramphenicol O-Acetyltransferase | Cloning, Molecular | DNA-Binding Proteins | Dihydrotestosterone | Flutamide | Humans | Male | Molecular Sequence Data | Nuclear Receptor Coactivators | Oncogene Proteins | Prostate | Prostatic Neoplasms | RNA, Messenger | Receptors, Androgen | Receptors, Steroid | Recombinant Proteins | Testosterone | Trans-Activators | Transcription Factors | Transcription, Genetic | Transfection | Tumor Cells, Cultured

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.