• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Activation of yeast protein kinase C by Rho1 GTPase.

We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of Pkc1.rho1 mutants are also impaired in pathway activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP-dependent manner. Recombinant Rho1 confers upon Pkc1 the ability to be stimulated by phosphatidylserine, indicating that Rho1 controls signal transmission through Pkc1.

Pubmed ID: 8621575


  • Kamada Y
  • Qadota H
  • Python CP
  • Anraku Y
  • Ohya Y
  • Levin DE


The Journal of biological chemistry

Publication Data

April 19, 1996

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM48533

Mesh Terms

  • Cell Cycle Proteins
  • Enzyme Activation
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • Guanosine Triphosphate
  • Models, Biological
  • Mutagenesis, Site-Directed
  • Protein Kinase C
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Temperature
  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
  • rho GTP-Binding Proteins