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Activation of yeast protein kinase C by Rho1 GTPase.

We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of Pkc1.rho1 mutants are also impaired in pathway activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP-dependent manner. Recombinant Rho1 confers upon Pkc1 the ability to be stimulated by phosphatidylserine, indicating that Rho1 controls signal transmission through Pkc1.

Pubmed ID: 8621575


  • Kamada Y
  • Qadota H
  • Python CP
  • Anraku Y
  • Ohya Y
  • Levin DE


The Journal of biological chemistry

Publication Data

April 19, 1996

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM48533

Mesh Terms

  • Cell Cycle Proteins
  • Enzyme Activation
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • Guanosine Triphosphate
  • Models, Biological
  • Mutagenesis, Site-Directed
  • Protein Kinase C
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Temperature
  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
  • rho GTP-Binding Proteins