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Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.

Hsubc9, a human gene encoding a ubiquitin-conjugating enzyme, has been cloned. The 18-kDa HsUbc9 protein is homologous to the ubiquitin-conjugating enzymes Hus5 of Schizosaccharomyces pombe and Ubc9 of Saccharomyces cerevisiae. The Hsubc9 gene complements a ubc9 mutation of S. cerevisiae. It has been mapped to chromosome 16p13.3 and is expressed in many human tissues, with the highest levels in testis and thymus. According to the Ga14 two-hybrid system analysis, HsUbc9 protein interacts with human recombination protein Rad51. A mouse homolog, Mmubc9, encodes an amino acid sequence that is identical to the human protein. In mouse spermatocytes, MmUbc9 protein, like Rad51 protein, localizes in synaptonemal complexes, which suggests that Ubc9 protein plays a regulatory role in meiosis.

Pubmed ID: 8610150

Authors

  • Kovalenko OV
  • Plug AW
  • Haaf T
  • Gonda DK
  • Ashley T
  • Ward DC
  • Radding CM
  • Golub EI

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

April 2, 1996

Associated Grants

  • Agency: PHS HHS, Id: 5R01H900272
  • Agency: NCI NIH HHS, Id: CA39238
  • Agency: NIGMS NIH HHS, Id: GM49779

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Chromosome Mapping
  • Chromosomes, Human
  • DNA-Binding Proteins
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Ligases
  • Male
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • Rad51 Recombinase
  • Recombinant Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Homology, Amino Acid
  • Spermatocytes
  • Synaptonemal Complex
  • Ubiquitin-Conjugating Enzymes
  • X Chromosome
  • Y Chromosome