Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Identification of a cellular protein that specifically interacts with the essential cysteine region of the HIV-1 Tat transactivator.

Virology | Feb 15, 1996

The Tat protein of the human immunodeficiency virus (HIV) is a powerful activator of HIV gene expression. Genetic and biochemical evidence suggests that one or more cellular cofactors may be important for Tat activity. We have used two-hybrid interactive cloning in yeast to identify a partial cDNA clone (clone 10) from a human B-lymphoblastoid library that specifically interacts with the N-terminal 31 amino acids of HIV-1 Tat which contains the essential cysteine-rich portion of the Tat activation domain. The encoded protein also binds to purified Tat in vitro. Mutation of single essential cysteine residues in Tat abolishes interaction between Tat and clone 10, suggesting that interaction with the encoded protein is important for Tat activity. We have identified the full-length cDNA for the Tat binding protein and shown that overexpression of the encoded protein, Tip60 (Tat interactive protein, 60 kDa), results in a fourfold augmentation of Tat transactivation of the HIV-1 promoter in transient expression assays without increasing the basal activity of the HIV promoter or activating the heterologous RSV promoter. These data together with the genetic and in vitro binding data support the notion that Tip60 might be a cofactor of Tat involved in the regulation of HIV gene expression.

Pubmed ID: 8607265 RIS Download

Mesh terms: Acetyltransferases | Amino Acid Sequence | Base Sequence | Cloning, Molecular | Conserved Sequence | Cysteine | DNA, Complementary | Escherichia coli | Gene Expression Regulation, Viral | Gene Products, tat | HIV-1 | HeLa Cells | Histone Acetyltransferases | Humans | Molecular Sequence Data | Point Mutation | Promoter Regions, Genetic | Protein Binding | Proteins | Saccharomyces cerevisiae | tat Gene Products, Human Immunodeficiency Virus

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIAID NIH HHS, Id: AI-29200
  • Agency: NIAID NIH HHS, Id: AI-29541
  • Agency: NIDDK NIH HHS, Id: R01-DK-46764

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.