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Essential role of Stat6 in IL-4 signalling.

Interleukin-4 (IL-4) is a pleiotropic lymphokine which plays an important role in the immune system. IL-4 activates two distinct signalling pathways through tyrosine phosphorylation of Stat6, a signal transducer and activator of transcription, and of a 170K protein called 4PS. To investigate the functional role of Stat6 in IL-4 signalling, we generated mice deficient in Stat6 by gene targeting. We report here that in the mutant mice, expression of CD23 and major histocompatibility complex (MHC) class II in resting B cells was not enhanced in response to IL-4. IL-4 induced B-cell proliferation costimulated by anti-IgM antibody was abolished. The T-cell proliferative response was also notably reduced. Furthermore, production of Th2 cytokines from T cells as well as IgE and IgG1 responses after nematode infection were profoundly reduced. These findings agreed with those obtained in IL-4 deficient mice or using antibodies to IL-4 and the IL-4 receptor. We conclude that Stat6 plays a central role in exerting IL-4 mediated biological responses.

Pubmed ID: 8602263


  • Takeda K
  • Tanaka T
  • Shi W
  • Matsumoto M
  • Minami M
  • Kashiwamura S
  • Nakanishi K
  • Yoshida N
  • Kishimoto T
  • Akira S



Publication Data

April 18, 1996

Associated Grants


Mesh Terms

  • Animals
  • Antibodies, Helminth
  • B-Lymphocytes
  • Cell Line
  • Cells, Cultured
  • Cytokines
  • Gene Targeting
  • Histocompatibility Antigens Class II
  • Immunoglobulin A
  • Immunoglobulin Class Switching
  • Immunoglobulin E
  • Immunoglobulin G
  • Interleukin-4
  • Lymphocyte Activation
  • Mice
  • Nippostrongylus
  • Receptors, IgE
  • STAT6 Transcription Factor
  • Signal Transduction
  • Spleen
  • T-Lymphocytes
  • T-Lymphocytes, Helper-Inducer
  • Trans-Activators