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Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Cell | Nov 3, 1995

http://www.ncbi.nlm.nih.gov/pubmed/8521478

Cyclooxygenases 1 and 2 (COX-1 and COX-2) are key enzymes in prostaglandin biosynthesis and the target enzymes for the widely used nonsteroidal anti-inflammatory drugs. To study the physiological roles of the individual isoforms, we have disrupted the mouse Ptgs1 gene encoding COX-1. Homozygous Ptgs1 mutant mice survive well, have no gastric pathology, and show less indomethacin-induced gastric ulceration than wild-type mice, even though their gastric prostaglandin E2 levels are about 1% of wild type. The homozygous mutant mice have reduced platelet aggregation and a decreased inflammatory response to arachidonic acid, but not to tetradecanoyl phorbol acetate. Ptgs1 homozygous mutant females mated to homozygous mutant males produce few live offspring. COX-1-deficient mice provide a useful model to distinguish the physiological roles of COX-1 and COX-2.

Pubmed ID: 8521478 RIS Download

Mesh terms: Animals | Anti-Inflammatory Agents, Non-Steroidal | Arachidonic Acid | Blotting, Northern | Blotting, Western | Cloning, Molecular | Dinoprostone | Female | Gastritis | Genetic Vectors | Homozygote | Indomethacin | Macrophages, Peritoneal | Male | Mice | Mice, Inbred C57BL | Mutation | Otitis Externa | Phenotype | Plasmids | Platelet Aggregation | Prostaglandin-Endoperoxide Synthases | Stomach Ulcer

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM20069
  • Agency: NHLBI NIH HHS, Id: HL49227

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