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The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen.

Cell | Jan 15, 1993

The DNA-dependent protein kinase (DNA-PK) phosphorylates Sp1 and several other nuclear proteins. Here, we show that Sp1 and the DNA-PK must be colocalized on the same DNA molecule for efficient phosphorylation to occur. Interestingly, we find that the DNA-PK binds to and is activated by the ends of DNA molecules. Furthermore, we show that the DNA binding properties of the DNA-PK are identical to those of Ku, a well-characterized human autoimmune antigen. We demonstrate that the DNA-PK can be fractionated into two components, one of which is Ku and the other of which is a polypeptide of approximately 350 kd. DNA cross-linking and coimmunoprecipitation studies indicate that the catalytic 350 kd DNA-PK component is directed to DNA by protein-protein interactions with Ku. The implications of the unusual DNA binding mode and multicomponent nature of the DNA-PK are discussed.

Pubmed ID: 8422676 RIS Download

Mesh terms: Antigens, Nuclear | Base Sequence | DNA | DNA Helicases | DNA, Single-Stranded | DNA-Binding Proteins | HeLa Cells | Humans | Ku Autoantigen | Molecular Sequence Data | Nuclear Proteins | Phosphorylation | Precipitin Tests | Protein Binding | Protein Kinases | Sp1 Transcription Factor