Disruption of the murine IL-4 gene blocks Th2 cytokine responses.
Murine T-helper clones are classified into two distinct subsets (Th1 and Th2) on the basis of their patterns of lymphokine secretion. Th1 clones secrete interleukin-2 (IL-2), tumour necrosis factor-beta (TNF-beta) and interferon-gamma (IFN-gamma), whereas Th2 clones secrete IL-4, IL-5 and IL-10 (ref. 1). These subsets are reciprocally regulated by IL-4, IL-10 and IFN-gamma and differentially promote antibody or delayed-type hypersensitivity responses. To evaluate whether IL-4 is required for mounting Th2 responses, we generated IL-4-mutant mice (IL-4-/-) and assessed the cytokine secretion pattern of T cells both from naive and Nippostrongylus brasiliensis infected mice. CD4+ T cells from naive IL-4-/- mice failed to produce Th2-derived cytokines after in vitro stimulation. The levels of Th2 cytokines IL-5, IL-9 and IL-10 from CD4+ T cells obtained after nematode infection were significantly reduced. The reduced IL-5 production in IL-4-/- mice correlated with reduced helminth-induced eosinophilia, which has been shown to be dependent on IL-5 in vivo. We conclude that IL-4 is required for the generation of the Th2-derived cytokines and that immune responses dependent on these cytokines are impaired.
Pubmed ID: 8384701 RIS Download
Alleles | Animals | Antibody Formation | Base Sequence | Cells, Cultured | Crosses, Genetic | Cytokines | DNA | Eosinophilia | Female | Gene Rearrangement | Heterozygote | Homozygote | Immunoglobulin A | Immunoglobulin E | Immunoglobulin G | Interferon-gamma | Interleukin-4 | Interleukins | Kanamycin Kinase | Lymph Nodes | Lymphocyte Activation | Male | Mice | Mice, Inbred Strains | Molecular Sequence Data | Mutation | Nippostrongylus | Oligodeoxyribonucleotides | Phosphotransferases | Restriction Mapping | Strongylida Infections | T-Lymphocyte Subsets | T-Lymphocytes, Helper-Inducer | Transcription, Genetic