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The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.

The retinoblastoma protein (p110RB) interacts with many cellular proteins in complexes potentially important for its growth-suppressing function. We have developed and used an improved version of the yeast two-hybrid system to isolate human cDNAs encoding proteins able to bind p110RB. One clone encodes a novel type 1 protein phosphatase catalytic subunit (PP-1 alpha 2), which differs from the originally defined PP-1 alpha by an amino-terminal 11-amino-acid insert. In vitro-binding assays demonstrated that PP-1 alpha isoforms preferentially bind the hypophosphorylated form of p110RB. Moreover, similar p110RB sequences are required for binding PP-1 alpha 2 and SV40 large T antigen. Cell cycle synchrony experiments revealed that this association occurs from mitosis to early G1. The implications of these findings on the regulation of both proteins are discussed.

Pubmed ID: 8384581

Authors

  • Durfee T
  • Becherer K
  • Chen PL
  • Yeh SH
  • Yang Y
  • Kilburn AE
  • Lee WH
  • Elledge SJ

Journal

Genes & development

Publication Data

April 29, 1993

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM-44664
  • Agency: NHGRI NIH HHS, Id: HG-00463
  • Agency: PHS HHS, Id: O5758

Mesh Terms

  • Antigens, Polyomavirus Transforming
  • Base Sequence
  • Cell Division
  • Cloning, Molecular
  • DNA, Fungal
  • DNA-Binding Proteins
  • Escherichia coli
  • Fungal Proteins
  • Gene Expression Regulation, Fungal
  • Genomic Library
  • Humans
  • Isoenzymes
  • Macromolecular Substances
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases
  • Protein Binding
  • Recombinant Fusion Proteins
  • Retinoblastoma Protein
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Substrate Specificity
  • Transcription Factors