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The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.

Genes & development | Apr 29, 1993

The retinoblastoma protein (p110RB) interacts with many cellular proteins in complexes potentially important for its growth-suppressing function. We have developed and used an improved version of the yeast two-hybrid system to isolate human cDNAs encoding proteins able to bind p110RB. One clone encodes a novel type 1 protein phosphatase catalytic subunit (PP-1 alpha 2), which differs from the originally defined PP-1 alpha by an amino-terminal 11-amino-acid insert. In vitro-binding assays demonstrated that PP-1 alpha isoforms preferentially bind the hypophosphorylated form of p110RB. Moreover, similar p110RB sequences are required for binding PP-1 alpha 2 and SV40 large T antigen. Cell cycle synchrony experiments revealed that this association occurs from mitosis to early G1. The implications of these findings on the regulation of both proteins are discussed.

Pubmed ID: 8384581 RIS Download

Mesh terms: Antigens, Polyomavirus Transforming | Base Sequence | Cell Division | Cloning, Molecular | DNA, Fungal | DNA-Binding Proteins | Escherichia coli | Fungal Proteins | Gene Expression Regulation, Fungal | Genomic Library | Humans | Isoenzymes | Macromolecular Substances | Molecular Sequence Data | Phosphoprotein Phosphatases | Protein Binding | Recombinant Fusion Proteins | Retinoblastoma Protein | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Substrate Specificity | Transcription Factors

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM-44664
  • Agency: NHGRI NIH HHS, Id: HG-00463
  • Agency: PHS HHS, Id: O5758

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