• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Mice lacking TdT: mature animals with an immature lymphocyte repertoire.

In adult animals, template-independent (or N) nucleotides are frequently added during the rearrangement of variable (V), diversity (D), and joining (J) segments of lymphocyte receptor genes, greatly enhancing junctional diversity. Receptor genes from adult mice carrying a mutation in the terminal deoxynucleotidyl transferase (TdT) gene have few N nucleotides, providing proof that this enzyme is essential for creating diversity. Unlike those from normal adults, receptor genes from adult mutant mice show extensive evidence of homology-directed recombination, suggesting that TdT blocks this process. Thus, switch-on of the TdT gene during the first week after birth provokes an even greater expansion of lymphocyte receptor diversity than had previously been thought.

Pubmed ID: 8356452

Authors

  • Gilfillan S
  • Dierich A
  • Lemeur M
  • Benoist C
  • Mathis D

Journal

Science (New York, N.Y.)

Publication Data

August 27, 1993

Associated Grants

None

Mesh Terms

  • Animals
  • Base Sequence
  • DNA Nucleotidylexotransferase
  • Gene Rearrangement, B-Lymphocyte
  • Gene Rearrangement, T-Lymphocyte
  • Genes, Immunoglobulin
  • Immunoglobulin Joining Region
  • Immunoglobulin Variable Region
  • Lymphocytes
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Nucleotides
  • Receptors, Antigen, T-Cell
  • Recombination, Genetic