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Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytes.

During the assembly of immunoglobulin and T cell receptor variable region genes from variable (V), diversity (D), and joining (J) segments, the germline-encoded repertoire is further diversified by processes that include the template-independent addition of nucleotides (N regions) at gene segment junctions. Terminal deoxynucleotidyl transferase (TdT)-deficient lymphocytes had no N regions in their variable region genes, which shows that TdT is responsible for N region addition. In addition, certain variable region genes appeared at increased frequency in TdT-deficient thymocytes, which indicates that N region addition also influences repertoire development by alleviating sequence-specific constraints imposed on the joining of particular V, D, and J segments.

Pubmed ID: 8356451


  • Komori T
  • Okada A
  • Stewart V
  • Alt FW


Science (New York, N.Y.)

Publication Data

August 27, 1993

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI20047

Mesh Terms

  • Animals
  • B-Lymphocytes
  • Base Sequence
  • DNA Nucleotidylexotransferase
  • DNA Nucleotidyltransferases
  • Gene Rearrangement, B-Lymphocyte
  • Gene Rearrangement, T-Lymphocyte
  • Genes, Immunoglobulin
  • Immunoglobulin Joining Region
  • Immunoglobulin Variable Region
  • Mice
  • Molecular Sequence Data
  • Nucleotides
  • Receptors, Antigen, T-Cell
  • T-Lymphocytes
  • VDJ Recombinases