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Complex formation between RAS and RAF and other protein kinases.

http://www.ncbi.nlm.nih.gov/pubmed/8327501

We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.

Pubmed ID: 8327501 RIS Download

Mesh terms: Base Sequence | Fungal Proteins | GTP-Binding Proteins | Guanosine Triphosphate | MAP Kinase Kinase 1 | Mitogen-Activated Protein Kinase Kinases | Molecular Sequence Data | Protein Kinases | Protein-Serine-Threonine Kinases | Protein-Tyrosine Kinases | Proto-Oncogene Proteins | Proto-Oncogene Proteins c-raf | Proto-Oncogene Proteins p21(ras) | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Schizosaccharomyces | Schizosaccharomyces pombe Proteins | ras Proteins