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Lethal skeletal dysplasia from targeted disruption of the parathyroid hormone-related peptide gene.

The parathyroid hormone-related peptide (PTHrP) gene was disrupted in murine embryonic stem cells by homologous recombination, and the null allele was introduced into the mouse germ line. Mice homozygous for the PTHrP null mutation died postnatally, probably from asphyxia, and exhibited widespread abnormalities of endochondral bone development. Histological examination revealed a diminution of chondrocyte proliferation, associated with premature maturation of chondrocytes and accelerated bone formation. Analysis of earlier developmental stages revealed that disturbance in cartilage growth preceded abnormal endochondral bone formation. There were no morphological abnormalities apparent in other tissues. These results provide direct evidence implicating PTHrP in normal skeletal development and serve to emphasize its potential involvement in human osteochondrodysplasias.

Pubmed ID: 8314082

Authors

  • Karaplis AC
  • Luz A
  • Glowacki J
  • Bronson RT
  • Tybulewicz VL
  • Kronenberg HM
  • Mulligan RC

Journal

Genes & development

Publication Data

February 1, 1994

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK11794
  • Agency: NHLBI NIH HHS, Id: HL37569

Mesh Terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Bone and Bones
  • Cartilage
  • Cloning, Molecular
  • Embryonic and Fetal Development
  • Gene Deletion
  • Genes
  • Genes, Lethal
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Osteochondrodysplasias
  • Parathyroid Hormone
  • Parathyroid Hormone-Related Protein
  • Proteins
  • Recombinant Fusion Proteins