FcR gamma chain deletion results in pleiotrophic effector cell defects.
The gamma subunit of immunoglobulin Fc receptors is an essential component of the high-affinity receptor for IgG (Fc gamma RIII) and is associated with the high-affinity receptor for IgG (Fc gamma RI) and the T cell receptor-CD3 complex. It is required for both receptor assembly and signal transduction. Targeted disruption of this subunit results in immunocompromised mice. Activated macrophages from gamma chain-deficient mice unexpectedly lack the ability to phagocytose antibody-coated particles, despite normal binding. Defects in NK cell-mediated antibody-dependent cytotoxicity and mast cell-mediated allergic responses are evident in these animals, establishing the indispensable role of FcRs in these responses. However, loss of gamma chain does not appear to perturb T cell development, since both thymic and peripheral T cell populations appear normal. These mice thus represent an important tool for evaluating the role of these receptors in humoral and cellular immune responses.
Pubmed ID: 8313472 RIS Download
Animals | Base Sequence | Blotting, Southern | Cells, Cultured | Chimera | Cytotoxicity, Immunologic | DNA | DNA Primers | Female | Flow Cytometry | Gene Deletion | Genotype | Interleukin-4 | Macromolecular Substances | Macrophages, Peritoneal | Male | Mast Cells | Mice | Mice, Inbred C57BL | Mice, Transgenic | Molecular Sequence Data | Passive Cutaneous Anaphylaxis | Phagocytosis | Polymerase Chain Reaction | Prostaglandin D2 | RNA, Messenger | Receptors, IgE | Receptors, IgG | Spleen | Stem Cells | T-Lymphocytes