Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Specific association between the human DNA repair proteins XPA and ERCC1.

http://www.ncbi.nlm.nih.gov/pubmed/8197174

Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.

Pubmed ID: 8197174 RIS Download

Mesh terms: DNA Damage | DNA Repair | DNA-Binding Proteins | Electrophoresis, Polyacrylamide Gel | Endonucleases | HeLa Cells | Humans | Protein Binding | Proteins | Xeroderma Pigmentosum Group A Protein

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIA NIH HHS, Id: AG11085
  • Agency: NCI NIH HHS, Id: CA52461

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.