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Specific association between the human DNA repair proteins XPA and ERCC1.

Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.

Pubmed ID: 8197174

Authors

  • Li L
  • Elledge SJ
  • Peterson CA
  • Bales ES
  • Legerski RJ

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

May 24, 1994

Associated Grants

  • Agency: NIA NIH HHS, Id: AG11085
  • Agency: NCI NIH HHS, Id: CA52461

Mesh Terms

  • DNA Damage
  • DNA Repair
  • DNA-Binding Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Endonucleases
  • HeLa Cells
  • Humans
  • Protein Binding
  • Proteins
  • Xeroderma Pigmentosum Group A Protein