Neutrophil and B cell expansion in mice that lack the murine IL-8 receptor homolog.
Interleukin-8 (IL-8) is a proinflammatory cytokine that specifically attracts and activates human neutrophils. A murine gene with a high degree of homology to the two known human IL-8 receptors was cloned and then deleted from the mouse genome by homologous recombination in embryonic stem (ES) cells. These mice, although outwardly healthy, had lymphadenopathy, resulting from an increase in B cells, and splenomegaly, resulting from an increase in metamyelocytes, band, and mature neutrophils. Thus, this receptor may participate in the expansion and development of neutrophils and B cells. This receptor was the major mediator of neutrophil migration to sites of inflammation and may provide a potential therapeutic target in inflammatory disease.
Pubmed ID: 8036519 RIS Download
Animals | B-Lymphocytes | Bone Marrow | Cell Movement | Chimera | Hematopoiesis, Extramedullary | Inflammation | Leukocyte Count | Lymph Nodes | Mice | Mice, Inbred C57BL | Neutrophils | Receptors, Interleukin | Receptors, Interleukin-8B | Recombination, Genetic | Spleen | Stem Cells