Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice.
We have used a retroviral gene trap in embryonic stem (ES) cells to derive a recessive embryonic lethal mouse strain, ROSA beta-geo5. Mutant embryos display an enlarged pericardial cavity, bradycardia, a dilated fourth ventricle in the brain, and die between embryonic days 11 and 12. Whereas heart development in the mutant embryos is extensive, the ventricular wall is abnormally thin with a reduced number of trabeculae. Cloning of the trapped gene indicates that proviral insertion creates a null mutation in the transcriptional enhancer factor 1 (TEF-1) gene. Although transcription of a number of muscle-specific genes believed to be TEF-1 targets appears normal, the defect in cardiogenesis is likely attributable to diminished transcription of one or several cardiac-specific genes.
Pubmed ID: 7958896 RIS Download
Amino Acid Sequence | Animals | Base Sequence | Cloning, Molecular | DNA Primers | DNA, Complementary | DNA-Binding Proteins | Embryo, Mammalian | Female | Fetal Death | Genes, Lethal | Heart Defects, Congenital | Male | Mice | Mice, Mutant Strains | Molecular Sequence Data | Mutation | Nuclear Proteins | Retroviridae | Stem Cells | Transcription Factors